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http://www.ericthecarguy.com/
I'm glad to have made this video as it was requested a couple of times. I hope to make a video just on bleeding at some point as that is probably the hardest part of the operation and there are a couple of ways to go about it. For now enjoy this one. Comments are welcome.

Stay dirty

ETCG

Due to factors beyond the control of EricTheCarGuy, it cannot guarantee against unauthorized modifications of this information, or improper use of this information.  EricTheCarGuy assumes no liability for property damage or injury incurred as a result of any of the information contained in this video. EricTheCarGuy recommends safe practices when working with power tools, automotive lifts, lifting tools, jack stands, electrical equipment, blunt instruments, chemicals, lubricants, or any other tools or equipment seen or implied in this video.  Due to factors beyond the control of EricTheCarGuy, no information contained in this video shall create any express or implied warranty or guarantee of any particular result.  Any injury, damage or loss that may result from improper use of these tools, equipment, or the information contained in this video is the sole responsibility of the user and not EricTheCarGuy.

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Throwing Transcription for a Loop: The Role of Chromatin Insulators in the 3D Nucleus

Air date: Wednesday, December 14, 2011, 3:00:00 PM
Time displayed is Eastern Time, Washington DC Local

Category: Wednesday Afternoon Lectures

Description: Interactions among regulatory sequences such as enhancers, promoters and insulators give rise to a three-dimensional structure of the genetic material in the eukaryotic nucleus. We have used 5C and HiC to determine the nature of inter- and intra-chromosomal interactions in the Drosophila genome. The results suggest that each chromosome arm is an independent physical unit in terms of its interactions with the rest of the genome. Each chromosome folds into modules with a hierarchical organization. These modules comprise 80% of the genome, they are composed of different types of chromatin, they have a low gene density, and the genes are expressed at low levels, independent of their chromatin composition. Chromosome modules are separated by inter-module regions that comprise 20% of the genome. Inter-module regions have high gene density and comprise different chromatin types, but genes are expressed at higher levels than in chromosome modules. Inter-chromosome modules contain insulator proteins, which, together with RNA polymerase II, mediate interactions with other inter-chromosome modules. Chromosome modules also interact with each other but these interactions are not enriched in any known proteins. We are attempting to deconstruct this 3D structure into specific classes of interactions and determine their role in gene expression. In addition, we are exploring mechanisms by which organization can be regulated. In particular, parylation of insulator proteins appears to regulate interactions among these proteins and the establishment of a specific three-dimensional organization of the chromatin.

The NIH Wednesday Afternoon Lecture Series includes weekly scientific talks by some of the top researchers in the biomedical sciences worldwide.

Author: Dr. Victor Corces, Emory University

Runtime: 01:05:56

Permanent link: http://videocast.nih.gov/launch.asp?17027

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